The four matches in the fourth round at Flushing Meadows on Monday in the final major of 2013 saw Williams, Li Na, Suarez Navarro and Makarova all advance at the expense of Sloane Stephens, Jelena Jankovic, Angelique Kerber and Agnieszka Radwanska.
Perhaps the shock of the day's results was Russian 24th-seed Makarova's 6-4, 6-4 win over the third-seeded Pole Radwanska.
Radwanska, who made the 2012 Wimbledon final, was expected to be one of the real championship threats at the US Open but she will now have to wait another year for a crack at her maiden Grand Slam title.
Makarova had already pulled off one upset downing this year's Wimbledon finalist Sabine Lisicki in the third round and now has beaten the last two losing finalists at the Championships in two straight matches at the US Open.
The Russian 25-year-old will now face China's Li Na in the quarter finals after the fifth-seed and 2011 French Open champion won her fourth round clash with former world No. 1 Serb Jankovic, 6-3, 6-0.
Neither player was having any luck whatsoever in holding serve throughout the first but Li was able to slightly improve her service as the match went on and Jankovic certainly didn’t as evidenced with the bagel of a second set.
Li was able to break Jankovic's serve seven times from only seven attempts with the Serb hitting four double-faults and able to win just 30 per cent of her first serves that landed in, and then a disastrous three from 14 of her second serves at a paltry 21 per cent.
With four double-faults, winning only three points on her second serve and hitting 22 unforced errors, Jankovic never gave herself a chance against the Chinese star.
World No. 1 Williams is also comfortably through to the quarter finals as she attempts to win her 17th career Grand Slam and fifth US Open title.
It loomed as a fascinating battle as she came up against fellow American and 15th-seed Stephens who knocked her out of the Australian Open in the quarter finals earlier this year, but on this occasion Williams had all the answers winning 6-4, 6-1.
That sets up a quarter-final match-up now for Williams up against Spanish veteran Suarez Navarro, who also recorded somewhat of a fourth round upset on Sunday beating the eighth-seeded German Kerber, 4-6, 6-3, 7-6 (3).
Attention now moves to Day 8 action and the other half of Round 4 women's action with four more enthralling matches to be on show.
First up on Arthur Ashe Stadium is the rejuvenated Slovak Daniela Hantuchova taking on rising American Alison Riske in the clash of two unseeded players looking to win through to an unlikely quarter-final appearance.
Next up on Arthur Ashe will be the battle of two former world No. 1 players when Serbia's Ana Ivanovic faces Belarusian Victoria Azarenka.
Italian veteran Flavia Pennetta will then look to keep her run going when she takes on Romania's 21st-seed Simona Halep on Louis Armstrong Stadium while Italian pair Roberta Vinci and Camila Giorgi also do battle on the Grandstand.