Australian Anti-Doping Authority agents fronted Bombers players at Essendon headquarters on Wednesday.
Bombers chairman David Evans welcomed the advancement in the controversial inquiry called by Essendon's hierarchy a little more than a fortnight ago.
Evans said the lecture from ASADA officials was a vital step in the overall process of a potentially lengthy and far-reaching investigation.
"This was an important step forward in helping to resolve the matters before the club," Evans said in a released Essendon statement.
"ASADA outlined the process for the investigation to continue, including interviews with players to determine if a prohibited substance had been administered.
Evans said the Bombers remain subject to the ASADA investigation and restritions in confidentiality.
The Bombers chief reiterated that his players and football staff will full cooperate with the ASADA investigation.
"I know our members and the football community want answers, and I ask that the club be allowed time to cooperate with the ongoing investigation," he said.
Evans concluded with an almost obligatory reminder that Essendon will make "no further comment" until the inquiry is complete.