Demetriou announced proposed increases in drug testing throughout the AFL.
He also declared plans for an immediate audit of all AFL clubs prescriptions and use of chemical supplements used on players in their training and rehabilitation programs.
The league boss and commission chairman Mike Fitzpatrick will meet with AFL club presidents, chief executives, coaches and football managers as early as next week.
Demetriou and Fiztpatrick threw the league's full support behind the expanded powers of the nation's highest crime fighters as well as Australia's Sports Anti-Doping Authority in an escalated war on illicit drugs in sport and the AFL.
Demetriou was adamant he could not reveal specific details of investigations already under way, especially the shock revelations that ASADA has launched an inquiry into alleged illicit substance use at Essendon.
Demetriou held a media conference back in Melbourne after he flanked Federal Government authorities at Parliament House in Canberra in announcing shock findings of widespread illegal drugs use throughout Australian sport, as well as potential match-fixing and betting rorts.
The nation's highest sports and crime fighting authorities have been empowered to investigate allegations and prospects of illegal behaviour across the various codes.
Startling revelations of illegal drug use, match-fixing and betting scandals rocked Australian sport just two days after an investigation was launched into alleged illicit substance abuse at AFL power club Essendon.
Demetriou declared that drug cheats and organised crime figures infiltrating the game will be caught, punished and rubbed out.
"We are going to attack this issue and throw all the resources behind it," Demetriou said.
He blatantly refused to discuss Essendon's investigation.
"We aim to clean this sport up to rid the game of incidious, infiltration of organised crime figures," Demetriou declaed.
"To rid the game of the use of drugs and rid the game of cocktails, to have a register of people who work in the sport.
"All these loopholes will be closed.
"If you think you can run the gauntlet, wake up to yourself because you will be caught.
"Every lead and every piece of information that we have been given will be thoroughly investigated and we will leave no stone unturned to get to the bottom of the truth."
Demetriou announced significant expansion in AFL powers to fight illicit and performance enhancing drugs use and anti-corruption measures in the fight to retain the competition's integrity.
"The AFL commission has endorsed a significant investment and increase powers and resources to the AFL integrity unit," he said.
"That will include an investment in more people, more investigators, more technology, more intelligence capability and more testing.
"We've also been instructed to instigate an audit of every club's use of supplements and other treatments and to do that in conjunction with ASADA."
The league chief also revealed that instructions have been issued to AFL medical commissioners to have urgent meetings with all club doctors in a review of practices.
"We will also be introducing mandatory reporting of doping activity or approaches to engage in doping, which is similar to those rules in place to gambling and match fixing," Demetriou disclosed.
"We will enhance a registration of all club staff and personnel who have contacted players including all sports scientists, high performance managers and there will be a appropriate background checks which includes registering your background qualifications, which sports you may have worked in.
"This will apply to all club personnel, not just to those who work in football departments."